QX 314 bromide

Name: QX 314 bromide
Brand: Tocris Bioscience
SKU: 1014
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 1014

Citations (32)

(1)

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Key Product Details

Description:	Na⁺ channel blocker
Chemical Name:	N-(2,6-Dimethylphenylcarbamoylmethyl)triethylammonium bromide
Purity:	≥99% (HPLC)
Molecular Weight:	343.31

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Biological Activity for QX 314 bromide

QX 314 bromide is a membrane impermeable quaternary derivative of lidocaine, a blocker of voltage-activated Na⁺ channels. Intracellular QX 314 bromide also inhibits calcium currents in hippocampal CA1 pyramidal neurons.

Technical Data for QX 314 bromide

M. Wt	343.31
Formula	C₁₆H₂₇N₂OBr
Storage	Desiccate at RT
Purity	≥99% (HPLC)
CAS Number	24003-58-5
PubChem ID	9884487
InChi Key	DLHMKHREUTXMCH-UHFFFAOYSA-N
Smiles	[Br-].CC[N+](CC)(CC)CC(=O)NC1=C(C)C=CC=C1C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for QX 314 bromide

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water		100

Preparing Stock Solutions for QX 314 bromide

The following data is based on the product molecular weight 343.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.91 mL	14.56 mL	29.13 mL
5 mM	0.58 mL	2.91 mL	5.83 mL
10 mM	0.29 mL	1.46 mL	2.91 mL
50 mM	0.06 mL	0.29 mL	0.58 mL

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References for QX 314 bromide

References are publications that support the biological activity of the product.

Talbot and Sayer Intracellular QX-314 inhibits calcium currents in hippocampal CA1 pyramidal neurons. J.Neurophysiol. 1996 PMID: 8890325
Perkins and Wong Intracellular QX-314 blocks the hyperpolarization activated inward current Iq in hippocampal CA1 pyramidal cells. J.Neurophysiol. 1995 PMID: 7760149
Alreja and Aghajanian QX-314 blocks the potassium but not the sodium dependent components of the opiate response in locus coeruleus neurons. Brain.Res. 1994 PMID: 8205485
Stichartz The inhibition of sodium currents in myelinated nerve by quaternary derivatives of lidocaine. J.Gen.Physiol. 1973 PMID: 4541340

Citations for QX 314 bromide (32)

Showing 1 - 10 of 32 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for QX 314 bromide include:

He et al. (2020) Nucleus Accumbens Tac1-Expressing Neurons Mediate Stress-Induced Anhedonia-like Behavior in Mice Cell rep. 33
PMID: 33147466
Spoljaric et al. (2019) KCC2-Mediated Cl- Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Cell Rep. 26:1073
PMID: 30699338
Li et al. (2019) TMEM16B regulates anxiety-related behavior and GABAergic neuronal signaling in the central lateral amygdala. Elife. 8
PMID: 31482844
Purkey et al. (2018) AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca²⁺-Permeable AMPA Receptors to Control LTP. Cell Rep. 25:974
PMID: 30355502
Ferrarese et al. (2018) Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Rep. 24:3455
PMID: 30257207
Acuña et al. (2016) Phosphorylation state-dependent modulation of spinal glycine receptors alleviates inflammatory pain. J Clin Invest. 126:2547
PMID: 27270175
Sametsky et al. (2015) Enhanced GABAA-Mediated Tonic Inhibition in Auditory Thalamus of Rats with Behavioral Evidence of Tinnitus. J Neurosci. 35:9369
PMID: 26109660
Xue et al. (2015) Structural elements that underlie Doc2β function during asynchronous synaptic transmission. Proc Natl Acad Sci U S A. 112:E4316
PMID: 26195798
McGee et al. (2015) Auxiliary Subunit GSG1L Acts to Suppress Calcium-Permeable AMPA Receptor Function. J Neurosci. 35:16171
PMID: 26658868
Puranam et al. (2015) Disruption of Fgf13 causes synaptic excitatory-inhibitory imbalance and genetic epilepsy and febrile seizures plus. Korean J Physiol Pharmacol. 35:8866
PMID: 26063919
Ahn et al. (2015) Characteristics of K(+) Outward Currents in the Cochlear Outer Hair Cells of Circling Mice within the First Postnatal Week. PLoS One. 19:383
PMID: 26170743
Lench et al. (2015) Differential Effects of D-Cycloserine and ACBC at NMDA Receptors in the Rat Entorhinal Cortex Are Related to Efficacy at the Co-Agonist Binding Site. Toxins (Basel). 10:e0133548
PMID: 26193112
Chen and Chesler (2015) Autocrine boost of NMDAR current in hippocampal CA1 pyramidal neurons by a PMCA-dependent, perisynaptic, extracellular pH shift. J Neurosci. 35:873
PMID: 25609607
Dale et al. (2014) Vort. disinhibits pyramidal cell function and enhances synaptic plasticity in the rat hippocampus. J Psychopharmacol. 28:891
PMID: 25122043
Gaffaney et al. (2014) Mutations that disrupt Ca²⁺-binding activity endow Doc2β with novel functional properties during synaptic transmission. Mol Biol Cell. 25:481
PMID: 24356452
Chuhma et al. (2014) DA neurons control striatal cholinergic neurons via regionally heterogeneous DA and glutamate signaling. Proc Natl Acad Sci U S A. 81:901
PMID: 24559678
He and Bausch (2014) Synaptic plasticity in glutamatergic and GABAergic neurotransmission following chronic Mem. treatment in an in vitro model of limbic epileptogenesis. BMC Neurosci. 77:379
PMID: 24184417
He et al. (2013) Synaptic and extrasynaptic plasticity in glutamatergic circuits involving dentate granule cells following chronic N-MthD.-aspartate receptor inhibition. J Neurosci. 109:1535
PMID: 23255721
Egawa et al. (2013) Optogenetic probing and manipulation of the calyx-type presynaptic terminal in the embryonic chick ciliary ganglion. PLoS One. 8:e59179
PMID: 23555628
Wu et al. (2013) Ovarian hormone loss impairs excitatory synaptic transmission at hippocampal CA3-CA1 synapses. J Neurosci. 33:16158
PMID: 24107948
Lerner et al. (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci. 30:2160
PMID: 20147543
Toyoda et al. (2009) Roles of the AMPA receptor subunit GluA1 but not GluA2 in synaptic potentiation and activation of ERK in the anterior cingulate cortex. Mol Pain. 5:46
PMID: 19664265
Kuroyanagi et al. (2009) Postsynaptic glutamate receptor δ family contributes to presynaptic terminal differentiation and establishment of synaptic transmission. J Neurophysiol. 106:4912
PMID: 19258455
Zhang et al. (2009) Tamox. mediated estrogen receptor activation protects against early impairment of hippocampal neuron excitability in an oxygen/glucose deprivation brain slice ischemia model. Brain Res. 1247:196
PMID: 18992727
Hong et al. (2008) Glutamatergic transmission is sustained at a later period of development of medial nucleus of the trapezoid body-lateral superior olive synapses in circling mice. J Neurosci. 28:13003
PMID: 19036993
Zhang et al. (2008) Resveratrol attenuates early pyramidal neuron excitability impairment and death in acute rat hippocampal slices caused by oxygen-glucose deprivation. Exp Neurol. 212:44
PMID: 18495119
Baldelli et al. (2007) Lack of synapsin I reduces the readily releasable pool of synaptic vesicles at central inhibitory synapses. J Neurosci. 27:13520
PMID: 18057210
Toyoda et al. (2007) Requirement of extracellular signal-regulated kinase/mitogen-activated protein kinase for long-term potentiation in adult mouse anterior cingulate cortex. Mol Pain. 3:36
PMID: 18053155
Gipson and Yeckel (2007) Coincident glutamatergic and cholinergic inputs transiently depress glutamate release at rat schaffer collateral synapses. J Neurophysiol. 97:4108
PMID: 17303811
Xu et al. (2006) Presynaptic regulation of the inhibitory transmission by GluR5-containing kainate receptors in spinal substantia gelatinosa. Mol Pain. 2:29
PMID: 16948848
Linnemann et al. (2006) Transient change in GABA(A) receptor subunit mRNA expression in Lurcher cerebellar nuclei during Purkinje cell degeneration. Neuron. 7:59
PMID: 16872511
Sur et al. (2003) N-desmethylclozapine, an allosteric agonist at muscarinic 1 receptor, potentiates N-MthD.-aspartate receptor activity. Proc Natl Acad Sci U S A. 100:13674
PMID: 14595031

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QX-314 bromide blocks action potentials propagation

Name: Anonymous

Species: Rat

Assay Type: In Vitro

Cell Line/Tissue: Hippocampus

QX-314 was used to block voltage-gated sodium channels, i.e. prevent action potential propagation. The compound develops its effect in 1 microM concentration, in use-dependent mode. Figure: EPSC evoked by electrical stimulation in hippocampal pyramidal neuron at positive membrane potential (+40 mV); QX-314 is added to intracellular solution.

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