HPI 1

Name: HPI 1
Brand: Tocris Bioscience
SKU: 3839

Catalog # 3839 | Tocris Bioscience a Bio-Techne Brand

Citations (1)

COA / SDS

Inhibits Hedgehog signaling

Key Product Details

Description:	Inhibits Hedgehog signaling
Chemical Name:	1,4,5,6,7,8-Hexahydro-4-(3-hydroxyphenyl)-7-(2-methoxyphenyl)-2-methyl-5-oxo-3-quinolinecarboxylic acid 2-methoxyethyl ester
Purity:	≥95% (HPLC)
Molecular Weight:	463.52

View Full Technical Details for HPI 1

Technical Data
Citations (1) / Reviews

Biological Activity for HPI 1

HPI 1 is a hedgehog (Hh) signaling inhibitor. Inhibits Sonic hedgehog (Shh)-, SAG- and Gli-induced Hh pathway activation in Shh-LIGHT2 cells (IC₅₀ values are 1.5, 1.5, 4 and 6 μM for Shh-, SAG-, Gli2- and Gli1-induced activation). Also inhibits Hh pathway activation in SmoM2-LIGHT cells (IC₅₀ = 2.5 μM); inhibits the proliferation of cerebellar granule neuron precursors expressing SmoM2. Does not inhibit Wnt signaling.

Technical Data for HPI 1

M. Wt	463.52
Formula	C₂₇H₂₉NO₆
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	599150-20-6
PubChem ID	2926768
InChi Key	ZXSWZQSYZYMZKS-UHFFFAOYSA-N
Smiles	CC2=C(C(OCCOC)=O)C(C3=CC(O)=CC=C3)C1=C(N2)CC(C4=C(OC)C=CC=C4)CC1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for HPI 1

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	46.35	100
	ethanol	46.35	100

Preparing Stock Solutions for HPI 1

The following data is based on the product molecular weight 463.52.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.16 mL	10.79 mL	21.57 mL
5 mM	0.43 mL	2.16 mL	4.31 mL
10 mM	0.22 mL	1.08 mL	2.16 mL
50 mM	0.04 mL	0.22 mL	0.43 mL

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References for HPI 1

References are publications that support the biological activity of the product.

Hyman Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proc.Natl.Acad.Sci.USA 2009 PMID: 19666565

Citations for HPI 1 (1)

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Citations are publications that use Bio-Techne products. Selected citations for HPI 1 include:

Ling et al. (2021) Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell. 28:1090-1104.e6
PMID: 33915081

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