FIPI

Name: FIPI
Brand: Tocris Bioscience
SKU: 3600
Rating: 5 (2 reviews)

Catalog # 3600 | Tocris Bioscience a Bio-Techne Brand

(2)

Citations (7)

COA / SDS

Phospholipase D inhibitor

Key Product Details

Description:	Phospholipase D inhibitor
Chemical Name:	N-[2-[4-(2,3-Dihydro-2-oxo-1H-benzimidazol-1-yl)-1-piperidinyl]ethy]-5-fluoro-1H-indole-2-carboxamide hydrochloride
Purity:	≥98% (HPLC)
Molecular Weight:	457.93

View Full Technical Details for FIPI

Biological Activity for FIPI

FIPI is a potent phosholipase D (PLD) inhibitor (IC₅₀ values are 20 and 25 nM for PLD₂ and PLD₁ respectively). Ameliorates PLD₂-driven suppression of membrane ruffling and cell spreading. Orally available.

Compound Libraries for FIPI

FIPI is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for FIPI

M. Wt	457.93
Formula	C₂₃H₂₄FN₅O₂.HCl
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	1781834-93-2
PubChem ID	90488864
InChi Key	BNMQCDOCYPGJJD-UHFFFAOYSA-N
Smiles	O=C(N2)N(C3CCN(CCNC(C4=CC5=C(C=CC(F)=C5)N4)=O)CC3)C1=C2C=CC=C1.Cl

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for FIPI

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	22.9	50

Preparing Stock Solutions for FIPI

The following data is based on the product molecular weight 457.93.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	4.37 mL	21.84 mL	43.67 mL
2.5 mM	0.87 mL	4.37 mL	8.73 mL
5 mM	0.44 mL	2.18 mL	4.37 mL
25 mM	0.09 mL	0.44 mL	0.87 mL

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References for FIPI

References are publications that support the biological activity of the product.

Su 5-fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol.Pharmcol.
Monovich Optimization of halopemide for phospholipase D2 inhibition. Bioorg.Med.Chem.Lett. 2007 PMID: 17317170

Citations for FIPI (7)

Showing 1 - 7 of 7 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for FIPI include:

Tove Irene et al. (2021) Diacylglycerol kinase and phospholipase D inhibitors alter the cellular lipidome and endosomal sorting towards the Golgi apparatus. Cell Mol Life Sci. 78:985-1009
PMID: 32447426
Lin and Liu (2019) Mechanical Stretch Induces mTOR Recruitment and Activation at the Phosphatidic Acid-Enriched Macropinosome in Muscle Cell. Front Cell Dev Biol. 7:78
PMID: 31139627
Sajal et al. (2019) Role of PLD-PKCζ signaling axis in p47phox phosphorylation for activation of NADPH oxidase by angiotensin II in pulmonary artery smooth muscle cells. Cell Biol Int. 43:678-694
PMID: 30977575
Pitt et al. (2017) Neuroprotective astrocyte-derived Ins/IGF-1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers. Mol Biol Cell. 28:2623
PMID: 28963439
Wang et al. (2016) The prostaglandin E₂-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation. PLoS Pathog.. 12:e1005803
PMID: 27482714
Bonnefond et al. (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. Int J Mol Sci. 20:535
PMID: 25627260
Pastor et al. (2014) Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs. J Lipid Res. 55:966
PMID: 24610889

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Customer Reviews for FIPI (2)

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great product

Name: Anonymous

Species: Rat

Assay Type: In Vitro

Cell Line/Tissue: Hippocampal neuron

100 μM treat 45min

PMID: PMID: 28963439
used this drug as an inhibitor

Name: Anonymous

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: macrophages

Pretreat mouse macrophage cells with this inhibitor prior to the bacterial stimulation.

PMID: 27482714

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Product Documents for FIPI

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.