Key Product Details
Description: | Degrades mutant FKBP12F36V fusion proteins; useful alternative to genetic methods for target validation |
Chemical Name: | 1-[(2S)-1-Oxo-2-(3,4,5-trimethoxyphenyl)butyl]-(2S)-2-piperidinecarboxylate (1R)-3-(3,4-dimethoxyphenyl)-1-[2-[2-[[6-[[2-(2,6-dioxo-3-piperidinyl)-2,3-dihydro-1,3-dioxo-1H-isoindol-4-yl]oxy]hexyl]amino]-2-oxoethoxy]phenyl]propyl ester |
Purity: | ≥98% (HPLC) |
Molecular Weight: | 1049.18 |
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Citations for dTAG-13 (4)
Citations are publications that use Bio-Techne products. Selected citations for dTAG-13 include:
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Ma et al.
(2023)
Engineered PROTAC-CID systems for mammalian inducible gene regulation
J Am Chem Soc.
2023;
PMID: 36626587 -
Orth-He et al.
(2023)
Protein folding stress potentiates NLRP1 and CARD8 inflammasome activation.
Cell Rep.
2023;
PMID: 36649711 -
Sahillioglu et al.
(2021)
CRASH-IT switch enables reversible and dose-dependent control of TCR and CAR T-cell function.
Cancer Immunol.Res..
9:999
PMID: 34193461 -
Kaji et al.
(2020)
Characterization of cereblon-dependent targeted protein degrader by visualizing the spatiotemporal ternary complex formation in cells
Sci.Rep..
10:3088
PMID: 32080280
There are no citations that match your criteria.
Customer Reviews for dTAG-13 (4)
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Species: MouseAssay Type: In VitroCell Line/Tissue: Murine cell linesI tested this product to degrade FFKBP12-Myc protein. It is working nicely.
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Species: HumanAssay Type: In VitroCell Line/Tissue: GliomaAssayed the degradation of FKBP-tagged-protein with HA tag in glioma cells using dTAG-13 at 500nM concentration for 0h, 4h, 6h and 24h time intervals. Untransfected cells has no HA signal and in transfected cells with dTAG-13 treatment lead to complete degradation of FKBP-tagged-protein in whole cell lysates
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Species: MouseAssay Type: In VitroCell Line/Tissue: mESCAssayed different dTag concentrations (50nm, 250nm, 500nm) for the knockdown of a FKBP-tagged chromatin-bound protein (180kDa). Whole cell lysate (RIPA) revealed no change to WT cells (150kDa) and considerable knockdown in all conditions after just an hour of treatment.
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Species: MouseAssay Type: In VitroCell Line/Tissue: E14 (mouse Embryonic Stem cells)Used dTAG-13 to degrade a transgene tagged with FKBM12-F36V and HA tags to visualize the protein.This is a western blot probed for HA tag to track degradation. All samples are treated with 500nM dTAG-13. Lanes are: 1) parental cell line, 2) control (no treatment), 3) 30 mins of treatment, 4) 1hr of treatment, 5) 2hrs of treatment, 6) 4hrs of treatment, 7) 6hrs of treatment. The constructs, as shown by the western, was degraded almost entirely (>98% quantified), within 30 mins. I have tested batch 2 of this product at conc. as low as 50nM and still just as effective. No apparent toxicity to cells. Extremely satisfied.
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