API-2

Tocris Bioscience, part of Bio-Techne | Catalog # 2151

(1)

Citations (64)

COA / SDS

Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral

Key Product Details

Description:	Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral
Alternative Names:	Triciribine,NSC 154020,TCN
Chemical Name:	1,5-Dihydro-5-methyl-1-β-D-ribofuranosyl-1,4,5,6,8-pentaazaacenaphthylen-3-amine
Purity:	≥98% (HPLC)
Molecular Weight:	320.31

View Full Technical Details for API-2

Technical Data
Citations / Reviews (1)

Biological Activity for API-2

API-2 is a selective inhibitor of Akt (protein kinase B) signaling; displays minimal inhibition of PKC, PKA, SGK and p38 pathways. Inhibits phosphorylation and activation of downstream targets of Akt including Bad, GSK-3β and AFX. Induces apoptosis and growth arrest in vitro, preferentially in human cancer cells with elevated levels of Akt. Potently and selectively inhibits growth of Akt-overexpressing tumors in mice. Inhibits DNA synthesis and displays antiviral activity against HIV-1 and -2.

Compound Libraries for API-2

API-2 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for API-2

M. Wt	320.31
Formula	C₁₃H₁₆N₆O₄
Storage	Store at RT
Purity	≥98% (HPLC)
CAS Number	35943-35-2
PubChem ID	65399
InChi Key	HOGVTUZUJGHKPL-HTVVRFAVSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C3=C(C4=NC=N3)C(C(N)=NN4C)=C2)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for API-2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	32.03	100
	DMSO	32.03	100

Preparing Stock Solutions for API-2

The following data is based on the product molecular weight 320.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.22 mL
5 mM	0.62 mL	3.12 mL	6.24 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

Molarity Calculator

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Reconstitution Calculator

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References for API-2

References are publications that support the biological activity of the product.

Ptak Phosphorylation of triciribine is necessary for activity against HIV type 1. AIDS Res.Hum.Retroviruses. 1998 PMID: 9788672
Yang Akt/protein kinase B signaling inhibitor-2, a selective small molecule inhibitor of Akt signaling with antitumour activity in cancer cells overexpressing Akt. Cancer Res. 2004 PMID: 15231645
Wotring Dual mechanisms of inhibition of DNA synthesis by triciribine. Cancer Res. 1990 PMID: 2379153