API-2

Tocris Bioscience a Bio-Techne Brand

Catalog # 2151

Citations (17)

(1)

COA / SDS

Catalog #

Availability

Size / Price

Qty

Key Product Details

Description:	Selective inhibitor of Akt/PKB signaling. Antitumor and antiviral
Alternative Names:	Triciribine, NSC 154020, TCN
Chemical Name:	1,5-Dihydro-5-methyl-1-β-D-ribofuranosyl-1,4,5,6,8-pentaazaacenaphthylen-3-amine
Purity:	≥99% (HPLC)
Molecular Weight:	320.31

View Full Technical Details for API-2

Complete Your Research

Biological Activity for API-2

API-2 is a selective inhibitor of Akt (protein kinase B) signaling; displays minimal inhibition of PKC, PKA, SGK and p38 pathways. Inhibits phosphorylation and activation of downstream targets of Akt including Bad, GSK-3β and AFX. Induces apoptosis and growth arrest in vitro, preferentially in human cancer cells with elevated levels of Akt. Potently and selectively inhibits growth of Akt-overexpressing tumors in mice. Inhibits DNA synthesis and displays antiviral activity against HIV-1 and -2.

Compound Libraries for API-2

API-2 is also offered as part of the Tocriscreen 2.0 Max, Tocriscreen Kinase Inhibitor Library and Tocriscreen Antiviral Library. Find out more about compound libraries available from Tocris.

Technical Data for API-2

M. Wt	320.31
Formula	C₁₃H₁₆N₆O₄
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	35943-35-2
PubChem ID	65399
InChi Key	HOGVTUZUJGHKPL-HTVVRFAVSA-N
Smiles	O[C@@H]1[C@@H](CO)O[C@@H](N2C3=C(C4=NC=N3)C(C(N)=NN4C)=C2)[C@@H]1O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for API-2

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	1eq. HCl	32.03	100
	DMSO	32.03	100

Preparing Stock Solutions for API-2

The following data is based on the product molecular weight 320.31.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.12 mL	15.61 mL	31.22 mL
5 mM	0.62 mL	3.12 mL	6.24 mL
10 mM	0.31 mL	1.56 mL	3.12 mL
50 mM	0.06 mL	0.31 mL	0.62 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for API-2

References are publications that support the biological activity of the product.

Yang Akt/protein kinase B signaling inhibitor-2, a selective small molecule inhibitor of Akt signaling with antitumour activity in cancer cells overexpressing Akt. Cancer Res. 2004 PMID: 15231645
Ptak Phosphorylation of triciribine is necessary for activity against HIV type 1. AIDS Res.Hum.Retroviruses. 1998 PMID: 9788672
Wotring Dual mechanisms of inhibition of DNA synthesis by triciribine. Cancer Res. 1990 PMID: 2379153

Citations for API-2 (17)

Showing 1 - 10 of 17 citations Showing All

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for API-2 include:

Yuan et al. (2019) IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development. Cell Death Dis. 10:63
PMID: 30683845
Suarez et al. (2017) The AKT inhibitor triciribine in combination with PTX has order-specific efficacy against Zfp217-induced breast cancer chemoresistance. Oncotarget. 8:108534
PMID: 29312549
Ali et al. (2017) Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1. BMC Cancer. 17:803
PMID: 29187162
Arqués et al. (2016) Tankyrase Inhibition Blocks Wnt/β-Catenin Pathway and Reverts Resistance to PI3K and AKT Inhibitors in the Treatment of Colorectal Cancer. Clin Cancer Res. 22:644
PMID: 26224873
Rosenberg et al. (2015) Targeting Glutamatergic Signaling and the PI3 Kinase Pathway to Halt Melanoma Progression. Biol Open. 8:42614
PMID: 25749171
Kana et al. (2015) PI3K Phosphorylation Is Linked to Improved Electrical Excitability in an In Vitro Engineered Heart Tissue Disease Model System. Tissue Eng Part A. 21:2379
PMID: 26120935
Kuo et al. (2014) Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by Ins. J Lipid Res. 55:919
PMID: 24565756
Shravah et al. (2014) PropF. mediates signal transducer and activator of transcription 3 activation and crosstalk with phosphoinositide 3-kinase/AKT. Am J Physiol Renal Physiol. 3:e29554
PMID: 25105067
Robertson et al. (2013) Sea urchin akt activity is Runx-dependent and required for post-cleavage stage cell division. Blood. 2:472
PMID: 23789095
Woo et al. (2012) Calpain-mediated processing of p53-associated parkin-like cytoplasmic protein (PARC) affects chemosensitivity of human ovarian cancer cells by promoting p53 subcellular trafficking. J Biol Chem. 287:3963
PMID: 22117079
Duan et al. (2011) mTOR generates an auto-amplification loop by triggering the βTrCP- and CK1α-dependent degradation of DEPTOR. JAKSTAT. 44:317
PMID: 22017877
Barber and Sentman (2011) NKG2D receptor regulates human effector T-cell cytokine production. Mol Cell. 117:6571
PMID: 21518928
Sunters et al. (2010) Mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor alpha-mediated control of Ins-like growth factor (IGF) I receptor sensitivity to Ambient IGF, leading to phosphatidylinositol 3-kinase/AKT-dependent Wnt/LRP5 receptor-i J Biol Chem. 285:8743
PMID: 20042609
Jackson et al. (2010) Control of cerebellar long-term potentiation by P-Rex-family guanine-nucleotide exchange factors and phosphoinositide 3-kinase. PLoS One. 5:e11962
PMID: 20694145
Liu et al. (2010) FRP inhibits ox-LDL-induced endothelial cell apoptosis through an Akt-NF-{kappa}B-Bcl-2 pathway and inhibits endothelial cell apoptosis in an apoE-knockout mouse model. Am J Physiol Endocrinol Metab. 299:E351
PMID: 20530739
Roselli et al. (2009) Disassembly of shank and homer synaptic clusters is driven by soluble beta-amyloid(1-40) through divergent NMDAR-dependent signalling pathways. PLoS One. 4:e6011
PMID: 19547699
Madhunapantula et al. (2007) PRAS40 deregulates apoptosis in malignant melanoma. Cancer Res. 67:3626
PMID: 17440074

There are no citations that match your criteria.

Showing 1 - 10 of 17 citations Showing All

Customer Reviews for API-2 (1)

5 out of 5

1 customer rating

5 stars

100%

4 stars

3 stars

2 stars

1 star

Customer Images

Showing 1 - 1 of 1 review Showing All

Filter By:

Attenuation by co-treatment of either PI3K inhibitor LY94002 or Akt inhibitor API-2

Name: Zhenmin Lei

Species: Mouse

Assay Type: In Vitro

Cell Line/Tissue: Ovarian theca cells

The isolated mouse ovarian theca cells were maintained in 12-well plates withRPMI-1640 medium. For determination of Lhcgr expression, thecells were rinsed three times with PBS the day before incubation with vehicle(DMSO), 740YP (50 mg/ml), 740YP + API-2 (7.5 mM), LH (50 ng/mL),or LH + API-2 for 24 h. For determination of Akt phosphorylation,PBS rinsed cells were incubated with vehicle (DMSO), or API-2 (7.5 mM) in serum-free RPMI-medium for 1 h before the addition of LH (50 ng/mL) for 30 min.

PMID: PMID: 28137614

There are no reviews that match your criteria.