7α,25-Dihydroxycholesterol
Tocris Bioscience, part of Bio-Techne | Catalog # 6277
Highly potent GPR183 (EBI2) agonist
Discontinued Product
6277 has been discontinued.
View all Orphan 7-TM Receptor Agonists products.
Key Product Details
Description
Highly potent GPR183 (EBI2) agonist
Product Description
7α,25-Dihydroxycholesterol is a highly potent GPR183 (EBI2) agonist (EC50 = 140 pM) and putative natural ligand for GPR183. Regulates migration of B and T cells expressing GPR183 in vivo. Desensitizes B cells and reduces their movement to splenic follicles.Product Specifications
Molecular Weight
418.65
Formula
C27H46O3
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(3β,7α)-Cholest-5-ene-3,7,25-triol
CAS Number
64907-22-8
PubChem ID
11954197
InChI Key
BQMSKLCEWBSPPY-IKVTXIKFSA-N
SMILES
O[C@H](C1)CC[C@@]2(C)C1=C[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCCC(C)(O)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.19 | 10 with gentle warming |
Preparing Stock Solutions
for 7α,25-Dihydroxycholesterol
The following data is based on the product molecular weight 418.65
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 23.89 mL | 119.43 mL | 238.86 mL |
| 0.5 mM | 4.78 mL | 23.89 mL | 47.77 mL |
| 1 mM | 2.39 mL | 11.94 mL | 23.89 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Hannedouche Oxysterols direct immune cell migration via EBI2. Nature. 2011 PMID: 21796212
- Liu Oxysterols direct B-cell migration through EBI2. Nature. 2011 PMID: 21796211
Product Specific Notices for 7α,25-Dihydroxycholesterol
For research use only
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