1-EBIO

Name: 1-EBIO
Brand: Tocris Bioscience
SKU: 1041
Rating: 5 (1 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 1041

Citations (16)

(1)

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Key Product Details

Description:	Epithelial K_Ca channel activator
Chemical Name:	1-Ethyl-2-benzimidazolinone
Purity:	≥99% (HPLC)
Molecular Weight:	162.19

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Biological Activity for 1-EBIO

1-EBIO is an activator of epithelial K_Ca channels, stimulates a large and sustained trans-epithelial Cl^- secretory response across T84 monolayers. Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes.

Compound Libraries for 1-EBIO

1-EBIO is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.

Technical Data for 1-EBIO

M. Wt	162.19
Formula	C₉H₁₀N₂O
Storage	Store at RT
Purity	≥99% (HPLC)
CAS Number	10045-45-1
PubChem ID	82320
InChi Key	CXUCKELNYMZTRT-UHFFFAOYSA-N
Smiles	CCN1C(=O)NC2=C1C=CC=C2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for 1-EBIO

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	ethanol	16.22	100
	DMSO	16.22	100

Preparing Stock Solutions for 1-EBIO

The following data is based on the product molecular weight 162.19.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	6.17 mL	30.83 mL	61.66 mL
5 mM	1.23 mL	6.17 mL	12.33 mL
10 mM	0.62 mL	3.08 mL	6.17 mL
50 mM	0.12 mL	0.62 mL	1.23 mL

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References for 1-EBIO

References are publications that support the biological activity of the product.

Kleger Modulation of calcium-activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker-like cells. Circulation. 2010 PMID: 20956206
Kusama Reduced hyperpolarisation in endothelial cells of rabbit aortic value following chronic nitroglycerine administration. Br.J.Pharmacol. 2005 PMID: 16056232
Ayotunde and Adeagbo 1-Ethyl-2-benzimidazolinone stimulates endothelial K_Ca channels and nitric oxide formation in rat mesenteric vessels. Eur.J.Pharmacol. 1999 PMID: 10497901
Devor Modulation of Cl^- secretion by benzimidazolones. I. Direct activation of a Ca²⁺ - dependent K⁺ channel. Am.J.Physiol. 1996 PMID: 8944721

Citations for 1-EBIO (16)

Showing 1 - 10 of 16 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for 1-EBIO include:

Bushart et al. (2018) Targeting potassium channels to treat cerebellar ataxia. Ann Clin Transl Neurol. 5:297
PMID: 29560375
Gamble-George et al. (2016) Cyclooxygenase-2 inhibition reduces stress-induced affective pathology. Elife. 5
PMID: 27162170
Wells et al. (2016) Thalamic reticular impairment underlies attention deficit in Ptchd1(Y/-) mice. Nature. 532:58
PMID: 27007844
Rau et al. (2015) Increased Basolateral Amygdala Pyramidal Cell Excitability May Contribute to the Anxiogenic Phenotype Induced by Chronic Early-Life Stress. BMC Dermatol. 35:9730
PMID: 26134655
Cuppoletti et al. (2014) Differentiation between human ClC-2 and CFTR Cl- channels with pharmacological agents. Am J Physiol Cell Physiol. 307:C479
PMID: 25009109
Benton et al. (2013) Iberiotoxin-sensitive and -insensitive BK currents in Purkinje neuron somata. J Neurophysiol. 109:2528
PMID: 23446695
Cuppoletti et al. (2013) Methadone but not mor. inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents. Cell Biochem Biophys. 66:53
PMID: 22918821
Choi et al. (2013) Contradictory Effects of Superoxide and Hydrogen Peroxide on KCa3.1 in Human Endothelial Cells. Eur J Immunol. 17:181
PMID: 23776393
Cruse et al. (2011) Functional KCa3.1 K+ channels are required for human fibrocyte migration. J Allergy Clin Immunol. 128:1303
PMID: 21872912
Haddock et al. (2011) Diet-induced obesity impairs endothelium-derived hyperpolarization via altered potassium channel signaling mechanisms. PLoS One. 6:e16423
PMID: 21283658
Lai et al. (2011) PRL-3 promotes the proliferation of LoVo cells via the upregulation of KCNN4 channels. Oncol Rep. 26:909
PMID: 21725609
Park et al. (2011) Globotriaosylceramide leads to K(Ca)3.1 channel dysfunction: a new insight into endothelial dysfunction in Fabry disease. Cardiovasc Res. 89:290
PMID: 20971723
Duffy et al. (2007) Adenosine closes the K+ channel KCa3.1 in human lung mast cells and inhibits their migration via the adenosine A2A receptor. Proc Natl Acad Sci U S A. 37:1653
PMID: 17474152
Kusama et al. (2005) Reduced hyperpolarization in endothelial cells of rabbit aortic valve following chronic nitroglycerine administration. Br J Pharmacol. 146:487
PMID: 16056232
Manaves et al. (2004) Calcium and Vitamin D increase mRNA levels for the growth control hIK1 channel in human epidermal keratinocytes but functional channels are not observed. Korean J Physiol Pharmacol. 4:7
PMID: 15200683
Banderali et al. (2004) New insights on the voltage dependence of the KCa3.1 channel block by internal TBA. J Gen Physiol. 124:333
PMID: 15452196

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Study on BK channel activity

Name: Eleanor Baxter

Species: Human

Assay Type: In Vitro

It activates Ca2+ activated K+ channels on epithelium. Our University lab uses it to study Big Conductance Channel channel opening.

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