Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge New

Name: Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge
Brand: ProteinSimple
SKU: SPCKB-NP-015772

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-NP-015772

COA

Simple Plex Non-Human Primate Fibronectin assay kit for use on the Ella instrument. Contains Cartridge, Sample Diluent SD06, and Wash Buffer. Sample type indicated by NP (Plasma/Serum).

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Key Product Details

Assay Length:	90 minutes
Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge Summary:	Simple Plex assay is for the detection of non-human primate Fibronectin in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Non-human Primate
Specificity	Natural and recombinant human Fibronectin and Fibronectin fragments aa 1-1908 and aa 607-1265.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Fibronectin assay

Inter-Assay Precision (Precision between assays) Replicates of each Fibronectin control were tested in multiple assays performed by at least three technicians using two lots of reagents for non-human primate Fibronectin

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	18	21
Mean (ng/mL)	2.66	120	2.38	106
Standard Deviation	0.064	4	0.23	7.74
CV%	2.4	3.3	9.7	7.3

Recovery

Recovery for the non-human primate Fibronectin assays was evaluated at three different spiked concentrations of Fibronectin protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	109	106-113

Linearity

Samples containing and/or spiked with high concentrations of Fibronectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Non-Human Primate Fibronectin 2nd Gen Cartridge

	Ella Automated ELISA Platform and Simple Plex Non-Human Primate Fibronectin Assay
	Simple Plex Non-Human Primate Fibronectin Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Non-Human Primate Fibronectin Assay. Assay range is 0.03-321 ng/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when immobilized on a surface.

Item	Value
Entrez Gene IDs	2335 (Human); 280794 (Bovine)
Alternate Names	CIG, ED-B, FINC, FN, FN1, FNZ, Fibronectin, GFND, GFND2, LETS, MSF, SMDCF, fibronectin 1