Simple Plex Mouse IL-5 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-MP-002846
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Mouse IL-5 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-5 assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-5 control were tested in multiple assays performed by at least three technicians using two lots of reagents for mouse IL-5.
Mouse Cell Culture Supernates, Mouse EDTA Plasma, Mouse Heparin Plasma, Mouse Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 47 | 46 |
| Mean (pg/mL) | 28.3 | 1593 | 24.8 | 1537 |
| Standard Deviation | 1.65 | 107 | 2.23 | 127 |
| CV% | 5.8 | 6.7 | 9.0 | 8.2 |
Recovery for Simple Plex Mouse IL-5 Cartridge
Recovery for the mouse IL-5 assays was evaluated at three different spiked concentrations of IL-5 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse EDTA Plasma (n=5) | 109 | 98-120 |
| Mouse Heparin Plasma (n=5) | 107 | 98-111 |
| Mouse Serum (n=3) | 106 | 101-115 |
Linearity
Samples containing and/or spiked with high concentrations of mouse IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Mouse IL-5 Cartridge
Simple Plex Mouse IL-5 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Mouse IL-5 Assay. Assay range is 0.45-4290 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-5
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-5 Products
Product Documents for Simple Plex Mouse IL-5 Cartridge
Product Specific Notices for Simple Plex Mouse IL-5 Cartridge
For research use only