Simple Plex Human uPAR Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-007370
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human uPAR Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one uPAR assay.
Inter-Assay Precision (Precision between assays) Replicates of each uPAR control were tested in multiple assays performed by at least three technicians using two lots of reagents for human uPAR.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 49 | 50 |
| Mean (pg/mL) | 120 | 6099 | 110 | 5748 |
| Standard Deviation | 6.80 | 261 | 8.51 | 558 |
| CV% | 5.7 | 4.3 | 7.7 | 9.7 |
Recovery for Simple Plex Human uPAR Cartridge
Recovery was not necessary as human uPAR shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of uPAR were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human uPAR Cartridge
Simple Plex Human uPAR Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human uPAR Assay. Assay range is 1.68-16,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: uPAR
The urokinase-type Plasminogen Activator Receptor (uPAR) is a GPI-anchored protein that binds to both the inactive and active forms of uPA. This interaction serves to localize and enhance the proteolytic activity of uPA, leading to increased conversion of Plasminogen to Plasmin. uPA also triggers uPAR-mediated signal transduction, activation of protein tyrosine kinases, gene expression, cell adhesion, and chemotaxis. uPAR can additionally regulate cell adhesion by direct binding to Vitronectin and preventing Integrins from binding to their ligands.
Long Name
Alternate Names
Gene Symbol
Additional uPAR Products
Product Documents for Simple Plex Human uPAR Cartridge
Product Specific Notices for Simple Plex Human uPAR Cartridge
For research use only