Simple Plex Human TRAIL/TNFSF10 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000536
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TRAIL/TNFSF10 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TRAIL assay.
Inter-Assay Precision (Precision between assays) Replicates of each TRAIL control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TRAIL.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 22 |
| Mean (pg/mL) | 59.6 | 2519 | 53.4 | 2654 |
| Standard Deviation | 3.90 | 87.8 | 3.43 | 145 |
| CV% | 6.5 | 3.5 | 6.4 | 5.5 |
Recovery for Simple Plex Human TRAIL/TNFSF10 Cartridge
Recovery for the human TRAIL assays was evaluated at three different spiked concentrations of TRAIL protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 111 | 104-119 |
| EDTA Plasma (n=4) | 82 | 77-89 |
| Heparin Plasma (n=4) | 81 | 74-85 |
| Serum (n=4) | 78 | 73-86 |
Linearity
Samples containing and/or spiked with high concentrations of human TRAIL were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human TRAIL/TNFSF10 Cartridge
 curve.png "Simple Plex Human TRAIL Assay Standard Curve")
Simple Plex Human TRAIL Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TRAIL Assay. Assay range is 2.62-4000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: TRAIL/TNFSF10
TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.
Long Name
Alternate Names
Gene Symbol
Additional TRAIL/TNFSF10 Products
Product Documents for Simple Plex Human TRAIL/TNFSF10 Cartridge
Product Specific Notices for Simple Plex Human TRAIL/TNFSF10 Cartridge
For research use only