Simple Plex Human TNF RII/TNFRSF1B Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000535
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TNF RII/TNFRSF1B Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 1 | 2 | 2 |
| n | 16 | 28 | 16 | 24 |
| Mean (pg/mL) | 13.8 | 12.4 | 622 | 588 |
| Standard Deviation | 0.613 | 1.09 | 33.0 | 39.0 |
| CV% | 4.4 | 8.8 | 5.3 | 6.6 |
Recovery for Simple Plex Human TNF RII/TNFRSF1B Cartridge
Recovery was not necessary as human TNF RII shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human TNF RII were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human TNF RII/TNFRSF1B Cartridge
Human TNF RII/TNFRSF1B Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: TNF RII/TNFRSF1B
TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.
Long Name
Alternate Names
Gene Symbol
Additional TNF RII/TNFRSF1B Products
Product Documents for Simple Plex Human TNF RII/TNFRSF1B Cartridge
Product Specific Notices for Simple Plex Human TNF RII/TNFRSF1B Cartridge
For research use only