Simple Plex Human TGF-beta 1 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001369
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human TGF-beta 1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Sample Values
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TGF-beta 1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each TGF-beta 1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TGF-beta 1.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 25 | 25 |
| Mean (pg/mL) | 111 | 5909 | 102 | 5235 |
| Standard Deviation | 6.47 | 316 | 11.1 | 477 |
| CV% | 5.8 | 5.4 | 10.9 | 9.1 |
Recovery for Simple Plex Human TGF-beta 1 Cartridge
Recovery for the human TGF-beta 1 assays was evaluated at three different spiked concentrations of TGF-beta 1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Platelet-poor EDTA Plasma (n=4) | 101 | 90-118 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 83-95 |
Linearity
Samples containing and/or spiked with high concentrations of human TFG-beta 1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human TGF-beta 1 Cartridge
Simple Plex Human TGF-beta 1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TGF-beta 1 Assay. Assay range is 20.8-12,684 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: TGF-beta 1
TGF-beta 1 (Transforming Growth Factor beta 1) is a pleiotrophic cytokine that regulates immune function, proliferation, and epithelial-mesenchymal transition. TGF-beta 1 associates with LAP to form a latent complex. It can be is activated from latency by plasmin, matrix metalloproteases, thrombospondin 1, and a subset of integrins. TGF-beta 1 signals through complexes of TGF-beta RII with TGF-beta RI/ALK-5 or ALK-1. TGF-beta signaling is modulated by the accessory receptors TGF-beta RIII/Betaglycan and Endoglin/CD105.
Long Name
Alternate Names
Gene Symbol
Additional TGF-beta 1 Products
Product Documents for Simple Plex Human TGF-beta 1 Cartridge
Product Specific Notices for Simple Plex Human TGF-beta 1 Cartridge
For research use only