Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000221
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 24 | 22 |
| Mean (pg/mL) | 141 | 7745 | 151 | 7857 |
| Standard Deviation | 2.40 | 231 | 17.7 | 287 |
| CV% | 1.7 | 3.0 | 11.7 | 3.6 |
Recovery for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
Recovery was not necessary as ST2 shows natural linearity data from a neat sample to a 1:16 dilution.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=3) | 103 | 101-106 |
Linearity
Samples containing and/or spiked with high concentrations of human ST2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
Human ST2 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: ST2/IL-33R
ST2, also known as IL-1R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
Long Name
Alternate Names
Gene Symbol
Additional ST2/IL-33R Products
Product Documents for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
Product Specific Notices for Simple Plex Human ST2/IL-33R/IL-1R4 Cartridge
For research use only