Simple Plex Human Reg3A Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000524

Simple Plex Human Reg3A assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

1.93 pg/mL

Assay Range

9.83-15000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Reg3A Cartridge Summary

Simple Plex assay for the detection of human Reg3A in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all Reg3A Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human Reg3A.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 24 22
Mean (pg/mL) 91.0 4305 82.1 4011
Standard Deviation 3.36 416 6.38 405
CV% 3.7 9.7 7.8 10.1

Recovery for Simple Plex Human Reg3A Cartridge

Recovery was not necessary as Reg3A shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Reg3A were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human Reg3A Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human Reg3A Cartridge

Human Reg3A Simple Plex Assay Standard Curve

Human Reg3A Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Reg3A

The Reg family constitutes a subset of the calcium-dependent C-type lectin superfamily. These small, secreted proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. Studies also indicate a role for Reg family members in tumor formation and indicate their potential for use as biomarkers of carcinogenesis.

Reg3A also named lithostathine 3 and pancreatitis-associated protein 2, belongs to the Regenerating gene family of C-type lectins. Reg3 is overexpressed during the acute phase of pancreatitis.

Long Name

Regenerating Islet-derived IIIa

Alternate Names

Lithostathine 3, PAP2, REG3A

Entrez Gene IDs

5068 (Human); 19694 (Mouse); 171162 (Rat)

Gene Symbol

REG3A

Additional Reg3A Products

Product Documents for Simple Plex Human Reg3A Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human Reg3A Cartridge

For research use only

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