Simple Plex Human Lipocalin-2/NGAL Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000509
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Lipocalin-2/NGAL Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 21 | 20 |
| Mean (pg/mL) | 69.3 | 3041 | 66.8 | 3000 |
| Standard Deviation | 4.08 | 214 | 4.78 | 189 |
| CV% | 5.9 | 7.0 | 7.2 | 6.3 |
Recovery for Simple Plex Human Lipocalin-2/NGAL Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 102-104 |
Linearity
Samples containing and/or spiked with high concentrations of human Lipocalin-2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human Lipocalin-2/NGAL Cartridge
Human Lipocalin-2/NGAL Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Lipocalin-2/NGAL
Lipocalin-2/NGAL (Neutrophil Gelatinase-associated Lipocalin) is a secreted carrier protein that acts in the innate immune response, differentiation, tumorigenesis, and cell survival. It is induced in macrophages, glial cells, and epithelial cells during inflammation, anemia, and hypoxia. Lipocalin-2 binds catecholate siderophores which are secreted by bacteria and are required for bacterial iron uptake. Lipocalin-2 functions as a bacteriostatic agent in the innate immune response by limiting the bacterial iron supply. Iron uptake by Lipocalin-2 into mammalian cells is important for the regulation of iron-sensitive gene transcription. In the kidney, Lipocalin-2-mediated iron trafficking is required for protection from renal injury.
Long Name
Alternate Names
Gene Symbol
Additional Lipocalin-2/NGAL Products
Product Documents for Simple Plex Human Lipocalin-2/NGAL Cartridge
Product Specific Notices for Simple Plex Human Lipocalin-2/NGAL Cartridge
For research use only