Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Name: Simple Plex Human IL-6 (2nd Gen) Assay Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-003028
Price: 415 USD

ProteinSimple a Bio-Techne Brand

Catalog # SPCKB-PS-003028

Citations (1)

Product Datasheet / COA

Simple Plex Human IL-6 2nd-gen assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Sample Type & Volume Required Per Well:	Bronchoalveolar Lavage Fluid (12500 nL), Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (12500 nL)
Sample Types:	Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid
Sensitivity:	0.28 pg/mL
Assay Range:	0.28 - 2,652 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human IL-6 (2nd Gen) Assay Cartridge Summary:	Simple Plex assay for the detection of human IL-6 in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF) on Ella automated benchtop ELISA.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Specifications for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Reactivity	Human
Specificity	Natural and recombinant human IL-6.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	37.9	1763	38.7	1709
Standard Deviation	0.804	108	3.95	163
CV%	2.1	6.1	10.2	9.6

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=6)	95	92-98
Heparin Plasma (n=6)	93	91-94
Serum (n=6)	94	86-98

Linearity

Samples containing and/or spiked with high concentrations of human IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the IL-6 assay. This automated IL-6 ELISA is validated to measure IL-6 in cell culture supernates (CCS), serum, plasma, bronchoalveolar lavage fluid (BALF), and cerebrospinal fluid (CSF).

Scientific Data Examples for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

	Human IL-6 Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Item	Value
Long Name	Interleukin 6
Entrez Gene IDs	3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names	B-cell differentiation factor, B-cell stimulatory factor 2, BSF-2, BSF2, CDF, CTL differentiation factor , HSF, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, MGI-2A, hybridoma growth factor, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6

Citations for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge (1)

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Citations are publications that use Bio-Techne products. Selected citations for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge include:

S Morselli, C Foschi, L Laghi, S Zagonari, G Patuelli, T Camboni, C Ceccarani, C Consolandi, ME Djusse, MF Pedna, A Marangoni, M Severgnini, V Sambri Torquetenovirus in pregnancy: Correlation with vaginal microbiome, metabolome and pro-inflammatory cytokines Frontiers in Microbiology, 2022;13(0):998849.. 998849
PMID: 36160242

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Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

ProteinSimple a Bio-Techne Brand

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Product Specifications for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Precision

Recovery

Linearity

Scientific Data Examples for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Preparation and Storage

Background: IL-6

Citations for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge (1)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Product Documents for Simple Plex Human IL-6 (2nd Gen) Assay Cartridge

Product Datasheets

COA

Certificate of Analysis

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