Simple Plex Human IL-5 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000229
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-5 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 10 | 10 |
| Mean (pg/mL) | 22.5 | 999 | 19.0 | 886 |
| Standard Deviation | 1.69 | 54.9 | 1.90 | 73.3 |
| CV% | 7.5 | 5.5 | 10.1 | 8.3 |
Recovery for Simple Plex Human IL-5 Cartridge
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 94 | 87-102 |
| Heparin Plasma (n=4) | 95 | 85-101 |
| Serum (n=4) | 99 | 90-105 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-5 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human IL-5 Cartridge
Human IL-5 Ella Assay Standard Curve
Preparation and Storage
Formulation
Shipping
Stability & Storage
Background: IL-5
IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-5 Products
Product Documents for Simple Plex Human IL-5 Cartridge
Product Specific Notices for Simple Plex Human IL-5 Cartridge
For research use only