Simple Plex Human IL-10 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000276
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-10 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 6 | 6 |
| Mean (pg/mL) | 33.0 | 1591 | 33.2 | 1681 |
| Standard Deviation | 1.55 | 95.1 | 2.43 | 120 |
| CV% | 4.6 | 6.0 | 7.1 | 7.1 |
Recovery for Simple Plex Human IL-10 Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=6) | 105 | 88-113 |
| Heparin Plasma (n=6) | 102 | 89-118 |
| Serum (n=6) | 94 | 88-98 |
Linearity
<div>Samples containing and/or spiked with high concentrations of human IL-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.</div>
Scientific Data Images for Simple Plex Human IL-10 Cartridge
Human IL-10 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-10
IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-10 Products
Product Documents for Simple Plex Human IL-10 Cartridge
Product Specific Notices for Simple Plex Human IL-10 Cartridge
For research use only