Simple Plex Human IL-1 alpha Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000503
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IL-1 alpha Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-1 alpha assay.
Inter-Assay Precision (Precision between assays) Replicates of each IL-1 alpha control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-1 alpha.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 15 | 15 |
| Mean (pg/mL) | 12.4 | 640 | 12.2 | 577 |
| Standard Deviation | 0.410 | 27.9 | 0.448 | 47.8 |
| CV% | 3.3 | 4.4 | 3.7 | 8.3 |
Recovery for Simple Plex Human IL-1 alpha Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 121 | 106-131 |
| EDTA Plasma (n=4) | 102 | 97-108 |
| Heparin Plasma (n=4) | 102 | 99-105 |
| Serum (n=4) | 104 | 97-112 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-1 alpha were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IL-1 alpha Cartridge
Simple Plex Human IL-1 alpha Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-1 alpha Assay. Assay range is 0.49-1800 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IL-1 alpha/IL-1F1
IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signalling.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IL-1 alpha/IL-1F1 Products
Product Documents for Simple Plex Human IL-1 alpha Cartridge
Product Specific Notices for Simple Plex Human IL-1 alpha Cartridge
For research use only