Simple Plex Human IgG Pan Specific Cartridge

Name: Simple Plex Human IgG Pan Specific Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-007587

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-007587

Simple Plex Human IgG Pan Specific assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD19, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	329 pg/mL
Assay Range:	371 - 90,560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IgG Pan Specific Cartridge Summary:	Simple Plex assay is for the detection of human IgG Pan Specific in serum and plasma (EDTA/Heparin) in Sample Diluent SD19.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human IgG Pan Specific Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human IgG Pan Specific Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human IgG Pan Specific.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IgG Pan Specific assay

Inter-Assay Precision (Precision between assays) Replicates of each IgG Pan Specific control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IgG Pan Specific

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	53	54
Mean (pg/mL)	848	40300	662	37163
Standard Deviation	41	846	82	2781
CV%	4.9	2.1	12.3	7.5

Linearity

Samples containing and/or spiked with high concentrations of IgG Pan Specific were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human IgG Pan Specific Cartridge

	Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IgG Pan Specific Assay. Assay range is 371-90,560 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Item	Value
Long Name	Immunoglobulin G
Alternate Names	IgG, Immunoglobulin G, ImmunoglobulinG

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human IgG Pan Specific Cartridge

Product Datasheets

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Protocols & Manuals

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