Simple Plex Human IFN-beta Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000934
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
1.03 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Assay Range
6.16-3760 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human IFN-beta Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 28 |
| Mean (pg/mL) | 13.5 | 551 | 11.2 | 507 |
| Standard Deviation | 1.27 | 45.9 | 1.38 | 57.9 |
| CV% | 9.4 | 8.3 | 12.3 | 11.4 |
Recovery for Simple Plex Human IFN-beta Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 97 | 91-104 |
| EDTA Plasma (n=4) | 92 | 84-101 |
| Heparin Plasma (n=4) | 80 | 76-86 |
| Serum (n=4) | 95 | 83-106 |
Linearity
Samples containing and/or spiked with high concentrations of human IFN-beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IFN-beta Cartridge
Human IFN-beta Ella Assay Standard Curve - CCS with Sample Diluent SD13
Human IFN-beta Ella Assay Standard Curve - Serum/Plasma with Sample Diluent SD10
Preparation and Storage
Shipping
Stability & Storage
Background: IFN-beta
IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
Long Name
Alternate Names
Gene Symbol
Additional IFN-beta Products
Product Documents for Simple Plex Human IFN-beta Cartridge
Product Specific Notices for Simple Plex Human IFN-beta Cartridge
For research use only