Simple Plex Human IFN-alpha (multi-subtype) Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002812
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human IFN-alpha (multi-subtype) Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IFN-alpha (multi-subtype) assay.
Inter-Assay Precision (Precision between assays) Replicates of each IFN-alpha (multi-subtype) control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IFN-alpha (multi-subtype).
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 46 | 48 |
| Mean (pg/mL) | 82.6 | 4775 | 84.2 | 4425 |
| Standard Deviation | 3.71 | 215 | 7.41 | 390 |
| CV% | 4.5 | 4.5 | 8.8 | 8.8 |
Recovery for Simple Plex Human IFN-alpha (multi-subtype) Cartridge
Recovery for the human IFN-alpha (multi-subtype) assays was evaluated at three different spiked concentrations of IFN-alpha (multi-subtype) protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 88 | 83-91 |
| Heparin Plasma (n=4) | 86 | 80-92 |
| Serum (n=4) | 88 | 84-90 |
Linearity
Samples containing and/or spiked with high concentrations of human IFN-alpha (multi-subtype) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human IFN-alpha (multi-subtype) Cartridge
 curve.png "Simple Plex Human IFN-alpha (multi-subtype) Assay Standard Curve")
Simple Plex Human IFN-alpha (multi-subtype) Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IFN-alpha (multi-subtype) Assay. Assay range is 1.46-13,870 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: IFN-alpha
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional IFN-alpha Products
Product Documents for Simple Plex Human IFN-alpha (multi-subtype) Cartridge
Product Specific Notices for Simple Plex Human IFN-alpha (multi-subtype) Cartridge
For research use only