Simple Plex Human HE4/WFDC2 Cartridge

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000542

Simple Plex Human HE4/WFDC2 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

7.39 pg/mL

Assay Range

13.1-20000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human HE4/WFDC2 Cartridge Summary

Simple Plex assay for the detection of human HE4 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all HE4/WFDC2 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Reactivity

Human

Specificity

Natural and recombinant human HE4.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 43 50
Mean (pg/mL) 109 5517 125 5576
Standard Deviation 8.32 185 7.43 226
CV% 7.6 3.4 6.0 4.1

Recovery for Simple Plex Human HE4/WFDC2 Cartridge

Recovery was not necessary as HE4 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human HE4 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Human HE4 Simple Plex Assay Linearity

Scientific Data Images for Simple Plex Human HE4/WFDC2 Cartridge

Human HE4 Simple Plex Assay Standard Curve

Human HE4/WFDC2 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HE4/WFDC2

The HE4 gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members

Long Name

WAP Four-disulfide Core Domain protein 2

Alternate Names

EDDM4, WAP5, WFDC2

Entrez Gene IDs

10406 (Human); 67701 (Mouse); 286888 (Rat)

Gene Symbol

WFDC2

Additional HE4/WFDC2 Products

Product Documents for Simple Plex Human HE4/WFDC2 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human HE4/WFDC2 Cartridge

For research use only

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