Simple Plex Human GDF-15 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000269
Need a different panel or sample type? Customize your cartridge here.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human GDF-15 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one GDF-15 assay.
Inter-Assay Precision (Precision between assays) Replicates of each GDF-15 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human GDF-15.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 19 | 18 |
| Mean (pg/mL) | 13.5 | 571 | 12.6 | 599 |
| Standard Deviation | 0.898 | 54.0 | 1.11 | 56.0 |
| CV% | 6.7 | 9.5 | 8.8 | 9.3 |
Recovery for Simple Plex Human GDF-15 Cartridge
Recovery for the human GDF-15 assays was evaluated at three different spiked concentrations of GDF-15 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 110-113 |
Linearity
Samples containing and/or spiked with high concentrations of human GDF-15 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human GDF-15 Cartridge
Simple Plex Human GDF-15 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human GDF-15 Assay. Assay range is 0.52-2000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: GDF-15
GDF-15 (Growth Differentiation Factor 15) is a dimeric TGF-beta superfamily cytokine that is highly expressed in placenta and brain. It promotes neuronal survival and regulates neuropeptide Y and pro-opiomelanocortin levels to suppress appetite. It is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. GDF-15 also inhibits cartilage formation and the production of TNF-alpha production from LPS-stimulated macrophages.
Long Name
Alternate Names
Gene Symbol
Additional GDF-15 Products
Product Documents for Simple Plex Human GDF-15 Cartridge
Product Specific Notices for Simple Plex Human GDF-15 Cartridge
For research use only