Simple Plex Human G-CSF Cartridge

Name: Simple Plex Human G-CSF Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002224

ProteinSimple a Bio-Techne Brand

Catalog # SPCKB-PS-002224

Simple Plex Cartridge Kit for quantitation of G-CSF. Includes wash buffer and sample diluent. For use with the Ella automated immunoassay system.

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Key Product Details

Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	3.13 pg/mL
Assay Range:	3.13 - 11,936 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human G-CSF Cartridge Summary:	Simple Plex assay for the detection of human G-CSF in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Specifications for Simple Plex Human G-CSF Cartridge

Reactivity	Human
Specificity	Natural and recombinant human G-CSF.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	16
Mean (pg/mL)	89.7	3851	84.8	3999
Standard Deviation	3.36	277	8.81	455
CV%	3.7	7.2	10.4	11.4

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	97	93-100
Heparin Plasma (n=4)	95	93-98
Serum (n=4)	101	98-106

Linearity

Samples containing and/or spiked with high concentrations of human G-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human G-CSF Cartridge

	Human G-CSF Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: G-CSF

G-CSF (granulocyte-colony stimulating factor) is a secreted glycoprotein that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte lineage cells. It also enhances M-CSF induced monocyte differentiation and the proliferation of Th2-inducing dendritic cells. G-CSF promotes the development of T cell immune tolerance as well as tissue recovery following myocardial infarction and cerebral ischemia. It signals through G-CSF R/CD114 on monocytes, neutrophils, megakaryocytes, platelets, myeloid progenitors, trophoblasts and placenta, endothelial cells, and various tumor cell types. Mutations in G-CSF R are associated with congenital neutropenia.

Item	Value
Long Name	Granulocyte Colony Stimulating Factor
Entrez Gene IDs	1440 (Human); 12985 (Mouse)
Alternate Names	C17orf33, CSF3, CSF3OS, Filgrastim, G-CSF, GCSF, GCSFlenograstim, Lenograstim, MGC45931, Pluripoietin, chromosome 17 open reading frame 33, colony stimulating factor 3 (granulocyte), granulocyte colony-stimulating factor

Citations for Simple Plex Human G-CSF Cartridge

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