Simple Plex Human FGF-23 Cartridge

Name: Simple Plex Human FGF-23 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-002231

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-002231

Simple Plex Human FGF-23 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	1.06 pg/mL
Assay Range:	3.78 - 36,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human FGF-23 Cartridge Summary:	Simple Plex assay for the detection of human FGF-23 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human FGF-23 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human FGF-23 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human FGF-23.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	29
Mean (pg/mL)	185	10098	187	10286
Standard Deviation	5.43	660	22.1	840
CV%	2.9	6.5	11.8	8.2

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	94	90-97
Heparin Plasma (n=4)	99	95-102
Serum (n=4)	102	94-108

Linearity

Samples containing and/or spiked with high concentrations of human FGF-23 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human FGF-23 Cartridge

	Human FGF-23 Simple Plex Assay Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FGF-23

FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.

Item	Value
Long Name	Fibroblast Growth Factor 23
Entrez Gene IDs	8074 (Human); 64654 (Mouse)
Alternate Names	ADHR, FGF-23, FGF23, HPDR2, HYPF, PHPTC, Tumor-derived hypophosphatemia-inducing factor, fibroblast growth factor 23, phosphatonin, tumor-derived hypophosphatemia inducing factor

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human FGF-23 Cartridge

Product Datasheets

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Protocols & Manuals

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