Simple Plex Human Fas Ligand Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-004009
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Fas Ligand Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Fas Ligand assay.
Inter-Assay Precision (Precision between assays) Replicates of each Fas Ligand control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Fas Ligand.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 1 |
| n | 16 | 16 | 29 | 29 |
| Mean (pg/mL) | 25.7 | 1226 | 1286 | 27.2 |
| Standard Deviation | 1.24 | 55.3 | 103 | 1.95 |
| CV% | 4.8 | 4.5 | 8.0 | 7.2 |
Recovery for Simple Plex Human Fas Ligand Cartridge
Recovery was not necessary as human Fas Ligand shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Fas Ligand were serially diluted with Sample Diluent to produce samples within the range of the assay.
Scientific Data Images for Simple Plex Human Fas Ligand Cartridge
Simple Plex Human Fas Ligand Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Fas Ligand Assay. Assay range is 0.868-3312 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Fas Ligand/TNFSF6
Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a transmembrane protein homotrimer that binds to Fas/CD95 and triggers apoptosis in the Fas-expressing cell. Fas Ligand also binds the soluble decoy receptor DcR3. It is expressed on activated Th1 cells, CD8+ cytotoxic T cells, and NK cells. A soluble form can be released which remains trimeric and retains the ability to bind Fas. Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites. Tumor cells can evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes. In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity.
Alternate Names
Gene Symbol
Additional Fas Ligand/TNFSF6 Products
Product Documents for Simple Plex Human Fas Ligand Cartridge
Product Specific Notices for Simple Plex Human Fas Ligand Cartridge
For research use only