Simple Plex Human FABP2/I-FABP Cartridge

Name: Simple Plex Human FABP2/I-FABP Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000361

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000361

Simple Plex Human FABP2/I-FABP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	5.66 pg/mL
Assay Range:	31.1 - 118,560 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human FABP2/I-FABP Cartridge Summary:	Simple Plex assay is for the detection of human Fatty Acid Binding Protein 2 (FABP2) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human FABP2/I-FABP Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human FABP2/I-FABP Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human FABP2.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one FABP2 assay

Inter-Assay Precision (Precision between assays) Replicates of each FABP2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human FABP2

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	47	47
Mean (pg/mL)	792	37335	777	36690
Standard Deviation	43	842	67	2714
CV%	5.4	2.3	8.6	7.4

Recovery

Recovery for the human FABP2 assays was evaluated at three different spiked concentrations of FABP2 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	102	95-109
Heparin Plasma (n=3)	102	100-106
Serum (n=4)	104	86-113

Linearity

Samples containing and/or spiked with high concentrations of human FABP2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human FABP2/I-FABP Cartridge

	Simple Plex Human FABP2 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human FABP2 Assay. Assay range is 31.1 - 118,560 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: FABP2/I-FABP

Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

Item	Value
Long Name	Fatty Acid-Binding Protein 2/Intestinal FABP
Entrez Gene IDs	2169 (Human); 25598 (Rat)
Alternate Names	FABP2, FABPI, Fatty acid-binding protein 2, I-FABP, I-FABPMGC133132, IFABP, Intestinal FABP, Intestinal-type fatty acid-binding protein, fatty acid binding protein 2, intestinal, fatty acid-binding protein, intestinal

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Product Documents for Simple Plex Human FABP2/I-FABP Cartridge

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