Simple Plex Human Enolase 2 Cartridge New
ProteinSimple, part of Bio-Techne | Catalog # SPCKC-PS-015516
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Enolase 2 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Enolase 2 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Enolase 2 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Enolase 2.
Cell Culture Supernates, Cerebrospinal Fluid, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 62 | 60 |
| Mean (pg/mL) | 218 | 10,573 | 275 | 12,549 |
| Standard Deviation | 17.5 | 506 | 27.5 | 1025 |
| CV% | 8.0 | 4.8 | 10.0 | 8.2 |
Recovery for Simple Plex Human Enolase 2 Cartridge
Recovery for the human Enolase 2 assays was evaluated at three different spiked concentrations of Enolase 2 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 103 | 102-105 |
| Cerebrospinal Fluid (n=5) | 111 | 99-121 |
| Platelet-poor EDTA Plasma (n=4) | 111 | 103-122 |
| Platelet-poor Heparin Plasma (n=4) | 109 | 89-119 |
| Serum (n=4) | 108 | 98-114 |
Linearity
Samples containing and/or spiked with high concentrations of Enolase 2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Enolase 2 Cartridge
Simple Plex Human Enolase 2 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Enolase 2 Assay. Assay range is 140-13,643 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Enolase 2/Neuron-specific Enolase
Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
Alternate Names
Gene Symbol
Additional Enolase 2/Neuron-specific Enolase Products
- All Products for Enolase 2/Neuron-specific Enolase
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- Enolase 2/Neuron-specific Enolase ELISA Kits
- Enolase 2/Neuron-specific Enolase Lysates
- Enolase 2/Neuron-specific Enolase Primary Antibodies
- Enolase 2/Neuron-specific Enolase Proteins and Enzymes
- Enolase 2/Neuron-specific Enolase Simple Plex
Product Documents for Simple Plex Human Enolase 2 Cartridge
Product Specific Notices for Simple Plex Human Enolase 2 Cartridge
For research use only