Simple Plex Human DPPIV/CD28 Cartridge New
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-016840
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human DPPIV/CD28 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one DPPIV/CD26 assay.
Inter-Assay Precision (Precision between assays) Replicates of each DPPIV/CD26 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human DPPIV/CD26.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 51 | 45 |
| Mean (pg/mL) | 1277 | 58647 | 1253 | 60185 |
| Standard Deviation | 95.6 | 5653 | 136 | 5877 |
| CV% | 7.5 | 9.6 | 10.9 | 9.8 |
Recovery for Simple Plex Human DPPIV/CD28 Cartridge
Recovery for the human DPPIV/CD26 assays was evaluated at three different spiked concentrations of DPPIV/CD26 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 111 | 105-118 |
| Urine (n=4) | 110 | 107-114 |
Linearity
Samples containing and/or spiked with high concentrations of DPPIV/CD26 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human DPPIV/CD28 Cartridge
Simple Plex Human DPPIV/CD26 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human DPPIV/CD26 Assay. Assay range is 52.4-200,000 pg/mL.
Simple Plex Human DPPIV/CD26 Assay Endogenous Levels
Functional LOD and functional LLOQ are calculated as the LOD and LLOQ of the assay multiplied by the minimum required dilution (MRD) for the sample type.Preparation and Storage
Shipping
Stability & Storage
Background: DPPIV/CD26
DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.
Long Name
Alternate Names
Gene Symbol
Additional DPPIV/CD26 Products
Product Documents for Simple Plex Human DPPIV/CD28 Cartridge
Product Specific Notices for Simple Plex Human DPPIV/CD28 Cartridge
For research use only