Simple Plex Human Dkk-1 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000485
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Dkk-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each Dkk-1 control was tested multiple times in one human DKK-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Dkk-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Dkk-1.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 16 | 35 |
| Mean (pg/mL) | 83.5 | 3657 | 80.1 | 3816 |
| Standard Deviation | 5.26 | 270 | 7.51 | 277 |
| CV% | 6.3 | 7.4 | 9.4 | 7.3 |
Recovery for Simple Plex Human Dkk-1 Cartridge
Recovery was not necessary as human Dkk-1 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Dkk-1 Cartridge
Simple Plex Human Dkk-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Dkk-1 Assay.Preparation and Storage
Shipping
Stability & Storage
Background: Dkk-1
Dkk-1 (Dickkopf related protein 1) is a secreted antagonist of Wnt/beta-Catenin signaling. It interacts with LRP-6, and internalized complexes of Dkk-1/LRP-6/Kremen 2 can downregulate Wnt signaling. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. In addition, Dkk-1 can act as either a tumor suppressor or promoter of metastasis.
Long Name
Alternate Names
Gene Symbol
Additional Dkk-1 Products
Product Documents for Simple Plex Human Dkk-1 Cartridge
Product Specific Notices for Simple Plex Human Dkk-1 Cartridge
For research use only