Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000375
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CXCL13/BLC/BCA-1 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BLC/BCA-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each BLC/BCA-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BLC/BCA-1.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 14 | 13 |
| Mean (pg/mL) | 41.0 | 1797 | 38.6 | 1772 |
| Standard Deviation | 1.40 | 102 | 2.41 | 85.6 |
| CV% | 3.4 | 5.7 | 6.2 | 4.8 |
Recovery for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
Recovery for the human BLC/BCA-1 assays was evaluated at three different spiked concentrations of BLC/BCA-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 83 | 76-87 |
| Heparin Plasma (n=4) | 78 | 76-83 |
| Serum (n=4) | 81 | 77-84 |
Linearity
Samples containing and/or spiked with high concentrations of human BLC/BCA-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
Simple Plex Human BLC/BCA-1 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BLC/BCA-1 Assay. Assay range is 1.6-6110 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CXCL13/BLC/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naive B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer's patch development.
Alternate Names
Gene Symbol
Additional CXCL13/BLC/BCA-1 Products
Product Documents for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
Product Specific Notices for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
For research use only