Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Name: Simple Plex Human CXCL13/BLC/BCA-1 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000375

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000375

Simple Plex Human CXCL13/BLC/BCA-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	0.37 pg/mL
Assay Range:	1.6 - 6,110 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Human CXCL13/BLC/BCA-1 Cartridge Summary:	Simple Plex assay is for the detection of human B-Lymphocyte Chemoattractant (BLC/BCA-1) in serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Technical Data
Citations

Product Specifications for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human BLC/BCA-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one BLC/BCA-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each BLC/BCA-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human BLC/BCA-1

Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	14	13
Mean (pg/mL)	41	1797	38.6	1772
Standard Deviation	1.4	102	2.41	85.6
CV%	3.4	5.7	6.2	4.8

Recovery

Recovery for the human BLC/BCA-1 assays was evaluated at three different spiked concentrations of BLC/BCA-1 protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	83	76-87
Heparin Plasma (n=4)	78	76-83
Serum (n=4)	81	77-84

Linearity

Samples containing and/or spiked with high concentrations of human BLC/BCA-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

	Simple Plex Human BLC/BCA-1 Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human BLC/BCA-1 Assay. Assay range is 1.6-6110 pg/mL.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL13/BLC/BCA-1

CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.

Entrez Gene IDs

10563 (Human); 55985 (Mouse)

Alternate Names

ANGIE, ANGIE2, B cell-attracting chemokine 1, B lymphocyte chemoattractant, B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1), B-lymphocyte chemoattractant, BCA-1, BCA-1CXC chemokine BLC, BCA1, BCA1B-cell chemoattractant, BLC, BLCSmall-inducible cytokine B13, BLR1L, C-X-C motif chemokine 13, CXCL13, SCYB13, SCYB13B-cell-attracting chemokine 1, chemokine (C-X-C motif) ligand 13, chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant), small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human CXCL13/BLC/BCA-1 Cartridge

Product Datasheets

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Protocols & Manuals

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