Simple Plex Human CXCL10/IP-10 Cartridge Best Seller
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000237
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CXCL10/IP-10 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CXCL10/IP-10 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CXCL10/IP-10 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CXCL10/IP-10.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 14 | 12 |
| Mean (pg/mL) | 5.56 | 283 | 5.49 | 297 |
| Standard Deviation | 0.347 | 14.3 | 0.212 | 11.7 |
| CV% | 6.1 | 5.0 | 3.9 | 3.9 |
Recovery for Simple Plex Human CXCL10/IP-10 Cartridge
Recovery for the human CXCL10/IP-10 assays was evaluated at three different spiked concentrations of CXCL10/IP-10 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 87 | 74-105 |
| Heparin Plasma (n=4) | 92 | 85-103 |
| Serum (n=4) | 93 | 80-107 |
| Urine (n=8) | 100 | 90-115 |
Linearity
Samples containing and/or spiked with high concentrations of human CXCL10/IP-10 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human CXCL10/IP-10 Cartridge
Simple Plex Human CXCL10/IP-10 Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CXCL10/IP-10 Assay. Assay range is 0.60-920 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CXCL10/IP-10/CRG-2
CXCL10/IP-10 is an ELR- angiostatic chemokine that interacts with CXCR3 to attract Th1 cells, eosinophils, monocytes, and NK cells to sites of inflammation. It is upregulated by activated T lymphocytes, neutrophils, splenocytes, keratinocytes, osteoblasts, astrocytes, endothelial cells, smooth muscle cells, and pancreatic beta cells. CXCL10 contributes to disease pathology in many chronic inflammatory disorders including rheumatoid arthritis, psoriasis, cancer, multiple sclerosis, diabetes, and cardiovascular disease.
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Simple Plex Human CXCL10/IP-10 Cartridge
Product Specific Notices for Simple Plex Human CXCL10/IP-10 Cartridge
For research use only