Simple Plex Human CD14 Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000386
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CD14 Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD14 assay.
Inter-Assay Precision (Precision between assays) Replicates of each CD14 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD14.
EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 31 | 31 |
| Mean (pg/mL) | 31.7 | 1308 | 28.1 | 1233 |
| Standard Deviation | 3.07 | 95.9 | 2.74 | 162 |
| CV% | 9.7 | 7.3 | 9.7 | 13.2 |
Recovery for Simple Plex Human CD14 Cartridge
Recovery was not necessary as human CD14 shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human CD14 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CD14 Cartridge
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD14 Assay. Assay range is 16.9-4130 pg/mL.
Preparation and Storage
Shipping
Stability & Storage
Background: CD14
CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.
Alternate Names
Gene Symbol
Additional CD14 Products
Product Documents for Simple Plex Human CD14 Cartridge
Product Specific Notices for Simple Plex Human CD14 Cartridge
For research use only