Simple Plex Human CCL5/RANTES Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-000231
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL5/RANTES Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CCL5/RANTES assay.
Inter-Assay Precision (Precision between assays) Replicates of each CCL5/RANTES control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CCL5/RANTES.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 28 | 24 |
| Mean (pg/mL) | 18.4 | 787 | 15.6 | 728 |
| Standard Deviation | 0.710 | 47.4 | 1.06 | 51.1 |
| CV% | 3.9 | 6.0 | 6.8 | 7.0 |
Recovery for Simple Plex Human CCL5/RANTES Cartridge
Recovery was not necessary as human CCL5/RANTES shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human RANTES were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human CCL5/RANTES Cartridge
Simple Plex Human CCL5/RANTES Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CCL5/RANTES Assay. Assay range is 0.68-2600 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL5/RANTES
CCL5/RANTES is a secreted beta-chemokine that plays a primary role in the inflammatory immune response through chemoattraction and activation of leukocytes that express CCR1, 3, 4, or 5. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1.
Alternate Names
Gene Symbol
Additional CCL5/RANTES Products
Product Documents for Simple Plex Human CCL5/RANTES Cartridge
Product Specific Notices for Simple Plex Human CCL5/RANTES Cartridge
For research use only