Simple Plex Human CCL18/PARC Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-001408
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human CCL18/PARC Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CCL18/PARC assay.
Inter-Assay Precision (Precision between assays) Replicates of each CCL18/PARC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CCL18/PARC.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 40 | 41 |
| Mean (pg/mL) | 7.63 | 378 | 7.07 | 367 |
| Standard Deviation | 0.552 | 23.0 | 0.497 | 41.6 |
| CV% | 7.2 | 6.1 | 7.0 | 11.3 |
Recovery for Simple Plex Human CCL18/PARC Cartridge
Recovery was not necessary as human CCL18/PARC shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of human CCL18 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human CCL18/PARC Cartridge
Simple Plex Human CCL18/PARC Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CCL18/PARC Assay. Assay range is 0.12-1120 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: CCL18/PARC
Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.
Additional CCL18/PARC Products
Product Documents for Simple Plex Human CCL18/PARC Cartridge
Product Specific Notices for Simple Plex Human CCL18/PARC Cartridge
For research use only