Simple Plex Human Caspase-1 Cartridge (2nd Gen)
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003613
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Caspase-1 Cartridge (2nd Gen) Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Caspase-1 assay.
Inter-Assay Precision (Precision between assays) Replicates of each Caspace-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Caspace-1.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 29 | 30 |
| Mean (pg/mL) | 2.30 | 117 | 2.28 | 125 |
| Standard Deviation | 0.104 | 4.09 | 0.259 | 12.2 |
| CV% | 4.5 | 3.5 | 11.4 | 9.8 |
Recovery for Simple Plex Human Caspase-1 Cartridge (2nd Gen)
Recovery for the human Caspace-1 assays was evaluated at three different spiked concentrations of Caspace-1 protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cerebrospinal Fluid (n=5) | 116 | 103-123 |
| EDTA Plasma (n=4) | 90 | 78-101 |
| Heparin Plasma (n=4) | 90 | 82-97 |
| Serum (n=4) | 91 | 83-97 |
Linearity
Samples containing and/or spiked with high concentrations of Caspace-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Caspase-1 Cartridge (2nd Gen)
Human Caspase-1 Simple Plex Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Caspace-1 Assay. Assay range is 0.11-422 pg/mLPreparation and Storage
Shipping
Stability & Storage
Background: Caspase-1
Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa and two 10 kDa subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine.
Additional Caspase-1 Products
Product Documents for Simple Plex Human Caspase-1 Cartridge (2nd Gen)
Product Specific Notices for Simple Plex Human Caspase-1 Cartridge (2nd Gen)
For research use only