Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Name: Simple Plex Human Caspase-1 Cartridge (2nd Gen)
Brand: ProteinSimple
SKU: SPCKB-PS-003613

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003613

Simple Plex Human Caspase-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Key Product Details

Assay Length:	80 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Cerebrospinal Fluid (25 uL)
Sensitivity:	0.04 pg/mL
Assay Range:	0.11 - 422 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid)
Simple Plex Human Caspase-1 Cartridge (2nd Gen) Summary:	Simple Plex assay is for the detection of human Caspase-1/ICE in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin, and cerebrospinal fluid (CSF) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Technical Data
Citations

Product Specifications for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human Caspase-1.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Caspase-1 assay

Inter-Assay Precision (Precision between assays) Replicates of each Caspace-1 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Caspace-1

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Cerebrospinal Fluid

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	30
Mean (pg/mL)	2.3	117	2.28	125
Standard Deviation	0.104	4.09	0.259	12.2
CV%	4.5	3.5	11.4	9.8

Recovery

Recovery for the human Caspace-1 assays was evaluated at three different spiked concentrations of Caspace-1 protein.

Sample Type	Average % Recovery	Range %
Cerebrospinal Fluid (n=5)	116	103-123
EDTA Plasma (n=4)	90	78-101
Heparin Plasma (n=4)	90	82-97
Serum (n=4)	91	83-97

Linearity

Samples containing and/or spiked with high concentrations of Caspace-1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

	Human Caspase-1 Simple Plex Assay Standard Curve Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Caspace-1 Assay. Assay range is 0.11-422 pg/mL

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Caspase-1

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. They are expressed as latent zymogens and are activated by an autoproteolytic mechanism or by processing by other proteases (frequently other caspases). Human caspases can be subdivided into three functional groups: cytokine activation (caspase-1, -4, -5, and -13), apoptosis initiation (caspase-2, -8, -9, -and -10), and apoptosis execution (caspase-3, -6, and -7).

Caspases are regulated by a variety of stimili, including APAF1, CFLAR/FLIP, NOL3/ARC, and members of the inhibitor of apoptosis (IAP) family such as BIRC1/NAIP, BIRC2/cIAP-1, BIRC3/cIAP-2, BIRC4/XIAP, BIRC5/Survivin, and BIRC7/Livin. IAP activity is modulated by DIABLO/SMAC or PRSS25/HTRA2/Omi. Cell-permeable and irreversible peptide inhibitors are also available for different caspases.

Item	Value
Entrez Gene IDs	834 (Human)
Alternate Names	CASP-1, CASP1, CASP1 nirs variant 1, Caspase-1, Caspase-1 subunit p10, Caspase-1 subunit p20, Caspase1, EC 3.4.22.36, EC:3.4.22.36, ICE, IL-1 beta-converting enzyme, IL-1BC, IL1B-convertase, IL1BC, IL1BCE, Interleukin-1 beta convertase, Interleukin-1 beta-converting enzyme, caspase 1, apoptosis-related cysteine peptidase, interleukin 1, beta, convertase, interleukin 1-B converting enzyme, p45

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Human Caspase-1 Cartridge (2nd Gen)

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