Simple Plex Human ASC Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-003913
Customize Your Simple Plex Cartridge
Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human ASC Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one ASC assay.
Inter-Assay Precision (Precision between assays) Replicates of each ASC control were tested in multiple assays performed by at least three technicians using two lots of reagents for human ASC.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 57 | 55 |
| Mean (pg/mL) | 65.2 | 3191 | 61.4 | 3062 |
| Standard Deviation | 3.21 | 170 | 6.23 | 294 |
| CV% | 4.9 | 5.3 | 10.1 | 9.6 |
Recovery for Simple Plex Human ASC Cartridge
Recovery was not necessary as human ASC shows 5 points of natural linearity.
Linearity
Samples containing and/or spiked with high concentrations of ASC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human ASC Cartridge
Simple Plex Human ASC Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human ASC Assay. Assay range is 5.24-8000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: ASC
ASC contains an N-terminal Pyrin domain and C-terminal CARD (Caspase-recruitment Domain), and is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli. Methylation of the ASC gene has been correlated with decreased protein expression in numerous types of cancer.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional ASC Products
Product Documents for Simple Plex Human ASC Cartridge
Product Specific Notices for Simple Plex Human ASC Cartridge
For research use only