Simple Plex Human Amyloid Beta (aa1-40) Cartridge
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-008817
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human Amyloid Beta (aa1-40) Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 35 | 36 |
| Mean (pg/mL) | 35.2 | 1582 | 34.6 | 1524 |
| Standard Deviation | 1.27 | 73.4 | 3.58 | 137 |
| CV% | 3.6 | 4.6 | 10.4 | 9.0 |
Recovery for Simple Plex Human Amyloid Beta (aa1-40) Cartridge
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 98 | 94-103 |
| EDTA Plasma (n=4) | 91 | 86-94 |
| Heparin Plasma (n=4) | 88 | 83-95 |
| Serum (n=4) | 93 | 87-104 |
Linearity
Samples containing and/or spiked with high concentrations of human Amyloid beta (aa1-40) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Human Amyloid Beta (aa1-40) Cartridge
Human Amyloid beta (aa1-40) Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Amyloid beta
Amyloid Precursor Protein (APP) is a type I transmembrane protein that is ubiquitously expressed on cell surfaces. It undergoes complex proteolytic processing and is cleaved by alpha-, beta-, and gamma-Secretases to generate soluble APP alpha, soluble APP beta, and Amyloid beta (A beta) fragments of several lengths. One of these fragments, A beta 42, generated by beta- and gamma-Secretase activities, has been implicated in Alzheimer's disease. Aberrantly high levels of this peptide form and accumulate in the brains of Alzheimer's disease patients to create the senile plaques characteristic of the disease.
Alternate Names
Gene Symbol
Additional Amyloid beta Products
Product Documents for Simple Plex Human Amyloid Beta (aa1-40) Cartridge
Product Specific Notices for Simple Plex Human Amyloid Beta (aa1-40) Cartridge
For research use only