Simple Plex HIV-1 Gag p24 Cartridge

Name: Simple Plex HIV-1 Gag p24 Cartridge
Brand: ProteinSimple
SKU: SPCKB-PS-000981
Price: 415 USD

ProteinSimple a Bio-Techne Brand

Catalog # SPCKB-PS-000981

Simple Plex Human HIV-1 Gag p24 assay kit for use on Ella automated benchtop ELISA. Contains cartridge, sample diluent SD30, and wash buffer.

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Sample Types:	Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity:	0.67 pg/mL
Assay Range:	3.93 - 15,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex HIV-1 Gag p24 Cartridge Summary:	Simple Plex assay for the detection of HIV Gag p24 in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) on Ella automated benchtop ELISA.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Product Specifications for Simple Plex HIV-1 Gag p24 Cartridge

Specificity	Natural and recombinant HIV-1 Gag p24.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	35	34
Mean (pg/mL)	105	4500	94.3	4074
Standard Deviation	0.37	237	9.64	377
CV%	7.1	5.3	10.2	9.3

Recovery

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	114	111-118
EDTA Plasma (n=4)	107	102-116
Heparin Plasma (n=4)	107	99-112
Serum (n=4)	111	109-113

Linearity

Samples containing and/or spiked with high concentrations of human HIV Gag p24 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Examples for Simple Plex HIV-1 Gag p24 Cartridge

	HIV Gag p24 Simple Plex Assay Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55^Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Item	Value
Long Name	Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs	155030 (Viral)
Alternate Names	CA, Capsid Protein, HIV-1 Gag p24, HIV1 Gag p24, Pr55(Gag)

Citations for Simple Plex HIV-1 Gag p24 Cartridge

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Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Ella from ProteinSimple, a Bio-Techne brand. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

Simple Plex HIV-1 Gag p24 Cartridge

ProteinSimple a Bio-Techne Brand

Key Product Details

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Product Specifications for Simple Plex HIV-1 Gag p24 Cartridge

Precision

Recovery

Linearity

Scientific Data Examples for Simple Plex HIV-1 Gag p24 Cartridge

Preparation and Storage

Background: HIV-1 Gag p24

Citations for Simple Plex HIV-1 Gag p24 Cartridge

Compatible Instruments

Ella - Automated Immunoassay Platform | ProteinSimple

Product Documents for Simple Plex HIV-1 Gag p24 Cartridge

Product Datasheets

COA

Certificate of Analysis

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