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Simple Plex HEK 293 HCP 3G Cartridge Best Seller

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-OT-007066

Simple Plex Host Cell Protein/HCP assay kit for use on Ella instrument. Contains cartridge, sample diluent SD19, and wash buffer.

Customize Your Simple Plex Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and add up to 8 analytes per cartridge.


Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Bioprocess Samples (25 µL)

Sensitivity

0.2 ng/mL

Assay Range

1.64-400 ng/mL (Bioprocess Samples)

Simple Plex HEK 293 HCP 3G Cartridge Summary

Simple Plex assay for the detection of Human Embryonic Kidney Host Cell Protein (HEK 293 HCP 3G) in bioprocess samples.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.

Bioprocess Samples

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 8 8 81 79
Mean (ng/mL) 3.16 147 3.36 141
Standard Deviation 0.380 7.3 0.48 14.9
CV% 12.0 5.0 14.3 10.6

Recovery for Simple Plex HEK 293 HCP 3G Cartridge

Recovery at two different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Bioprocess Samples (n=12) 111 100-123

Linearity

Samples containing and/or spiked with high concentrations of HEK 293 HCP 3G were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Simple Plex HEK 293 HCP 3G Assay Linearity

Scientific Data Images for Simple Plex HEK 293 HCP 3G Cartridge

Simple Plex HEK 293 HCP 3G Assay Standard Curve

Simple Plex HEK 293 HCP 3G Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex HEK 293 HCP 3G Assay. Assay range is 1.64-400 ng/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Host Cell Protein/HCP

Long Name

Host Cell Protein

Additional Host Cell Protein/HCP Products

Product Documents for Simple Plex HEK 293 HCP 3G Cartridge

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Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex HEK 293 HCP 3G Cartridge

For research use only

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