Simple Plex Discovery Human Uromodulin Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014653
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Uromodulin Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Uromodulin assay.
Inter-Assay Precision (Precision between assays) Replicates of each Uromodulin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Uromodulin.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 36 | 36 |
| Mean (pg/mL) | 487 | 24539 | 493 | 23178 |
| Standard Deviation | 9.33 | 870 | 31.1 | 1206 |
| CV% | 1.9 | 3.5 | 6.3 | 5.2 |
Recovery for Simple Plex Discovery Human Uromodulin Assay
Recovery for the human Uromodulin assays was evaluated at three different spiked concentrations of Uromodulin protein.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 96 | 92-101 |
Linearity
Samples containing and/or spiked with high concentrations of Uromodulin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Uromodulin Assay
Simple Plex Discovery Human Uromodulin Assay Standard Curve
Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Discovery Human Uromodulin Assay. Assay range is 16.8-64,000 pg/mL.Preparation and Storage
Shipping
Stability & Storage
Background: Uromodulin
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85 - 95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-α and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Human Uromodulin is GPI-linked. Human Uromodulin is 78% aa identical to mouse Uromodulin.
Alternate Names
Gene Symbol
Additional Uromodulin Products
Product Documents for Simple Plex Discovery Human Uromodulin Assay
Product Specific Notices for Simple Plex Discovery Human Uromodulin Assay
For research use only