Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

Name: Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay
Brand: ProteinSimple
SKU: SPCKB-PS-014623

ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-014623

Simple Plex Human TRANCE assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Key Product Details

Assay Length:	90 minutes
Sample Type & Volume Required Per Well:	Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity:	3.1 pg/mL
Assay Range:	21 - 32,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay Summary:	Simple Plex Discovery assay is for the detection of human TNF-Related Activin-induced Cytokine (TRANCE) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View Full Technical Details for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

Technical Data
Citations

Product Specifications for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

Assay Type	Automated sandwich ELISA
Format	Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Reactivity	Human
Specificity	Natural and recombinant human TRANCE.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one TRANCE assay

Inter-Assay Precision (Precision between assays) Replicates of each TRANCE control were tested in multiple assays performed by at least three technicians using two lots of reagents for human TRANCE

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	24	24
Mean (pg/mL)	198	9796	253	12325
Standard Deviation	8.65	482	28.1	1286
CV%	4.4	4.9	11.1	10.4

Recovery

Recovery for the human TRANCE assays was evaluated at three different spiked concentrations of TRANCE protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	112	107-117
EDTA Plasma (n=2)	98	81-116
Heparin Plasma (n=2)	114	109-120
Serum (n=2)	108	101-115

Linearity

Samples containing and/or spiked with high concentrations of human TRANCE were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Examples for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

	Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human TRANCE Assay. Assay range is 21.0-32,000 pg/mL.

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRANCE/TNFSF11/RANK L

TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.

Item	Value
Long Name	TNF-related Activation-induced Cytokine
Entrez Gene IDs	8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names	CD254, CD254 antigen, ODF, OPGL, OPGLOPTB2, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK L, RANKL, RANKLreceptor activator of nuclear factor kappa B ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, TRANCEODFhRANKL2, sOdf, tumor necrosis factor (ligand) superfamily, member 11, tumor necrosis factor ligand superfamily member 11

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Ella Instrument. Get single or multiplex immunoassay results in less than 90 minutes that are consistent and highly reproducible.

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Product Documents for Simple Plex Discovery Human TRANCE/RANK L/TNFSF11 Assay

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