Simple Plex Discovery Human Pancreatic Polypeptide Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012305
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Pancreatic Polypeptide Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 42 | 42 |
| Mean (pg/mL) | 17.7 | 875 | 16.3 | 805 |
| Standard Deviation | 0.930 | 31.3 | 1.64 | 71.3 |
| CV% | 5.3 | 3.6 | 10.1 | 8.9 |
Recovery for Simple Plex Discovery Human Pancreatic Polypeptide Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 97 | 94-101 |
| EDTA Plasma (n=2) | 101 | 95-107 |
| Heparin Plasma (n=2) | 86 | 85-86 |
| Serum (n=2) | 91 | 87-95 |
Linearity
Samples containing and/or spiked with high concentrations of human Pancreatic Polypeptide were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Pancreatic Polypeptide Assay
Simple Plex Discovery Human Pancreatic Polypeptide/PP Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Pancreatic Polypeptide/PP
Pancreatic polypeptide (PP) is an ~4 kDa, unglycosylated member of the Neuropeptide-Y family of secreted peptide hormones. Human PP is synthesized with a 29 amino acid (aa) signal sequence and a 66 aa prohormone that contains the PP hormone, an icosapeptide of unknown function, and a C-terminal prosequence. PP is produced by pancreatic islet F-cells and released to the circulation following a meal. It slows stomach emptying time and insulin secretion and is thought to inhibit further food intake.
Additional Pancreatic Polypeptide/PP Products
Product Documents for Simple Plex Discovery Human Pancreatic Polypeptide Assay
Product Specific Notices for Simple Plex Discovery Human Pancreatic Polypeptide Assay
For research use only