Simple Plex Discovery Human IL-16 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012153
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human IL-16 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 22 | 22 |
| Mean (pg/mL) | 28.7 | 1349 | 23.1 | 1130 |
| Standard Deviation | 0.926 | 72.0 | 1.91 | 85.2 |
| CV% | 3.2 | 5.3 | 8.2 | 7.5 |
Recovery for Simple Plex Discovery Human IL-16 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 107 | 106-109 |
Linearity
Samples containing and/or spiked with high concentrations of human IL-16 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human IL-16 Assay
Simple Plex Discovery Human IL-16 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: IL-16
Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.
Long Name
Alternate Names
Gene Symbol
Additional IL-16 Products
Product Documents for Simple Plex Discovery Human IL-16 Assay
Product Specific Notices for Simple Plex Discovery Human IL-16 Assay
For research use only