Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012421
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 23 | 23 |
| Mean (pg/mL) | 225 | 9833 | 230 | 10003 |
| Standard Deviation | 5.96 | 314 | 25.0 | 601 |
| CV% | 2.6 | 3.2 | 10.9 | 6.0 |
Recovery for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 96 | 94-99 |
Linearity
Samples containing and/or spiked with high concentrations of human Galectin-3BP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay
Simple Plex Discovery Human Galectin-3BP/MAC-2BP Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Galectin-3BP/MAC-2BP
Galectin-3 binding protein, also known as MAC-2BP, M2BP, and 90K, is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily. Galectin-3BP binds Galectin-3 with high affinity, and additionally binds Galectins -1 and -7, several Collagen types, Fibronectin, beta 1 Integrins, and Nidogen. It is widely expressed in extracellular fluids and in pericellular areas of cell-rich tissues. Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers. It has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity. Galectin-3BP upregulation has also been correlated with tumor aggressiveness.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
Additional Galectin-3BP/MAC-2BP Products
Product Documents for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay
Product Specific Notices for Simple Plex Discovery Human Galectin-3BP/MAC-2BP Assay
For research use only