Simple Plex Discovery Human DLL1 Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012304
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human DLL1 Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple runs performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 34 | 34 |
| Mean (pg/mL) | 66.6 | 3137 | 70.2 | 3153 |
| Standard Deviation | 2.09 | 85.9 | 7.05 | 269 |
| CV% | 3.1 | 2.7 | 10.1 | 8.5 |
Recovery for Simple Plex Discovery Human DLL1 Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 120 | 118-121 |
Linearity
Samples containing and/or spiked with high concentrations of human DLL1 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human DLL1 Assay
Simple Plex Discovery Human DLL1 Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: DLL1
DLL1 (Delta-like protein 1) is a transmembrane glycoprotein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. It plays a role in central nervous system morphogenesis, intervertebral joint formation, inner ear hair cell development and patterning, and arteriogenesis. It regulates the homeostasis and differentiation of neural, pancreatic, and myogenic progenitor cells, T and B lymphocytes. DLL1 is overexpressed in a number of cancers and contributes to tumorigenesis. The extracellular region of DLL1 can be shed from the cell surface, and the intracellular domain can be cleaved by gamma-secretase to translocate to the nucleus and regulate gene transcription.
Long Name
Alternate Names
Gene Symbol
Additional DLL1 Products
Product Documents for Simple Plex Discovery Human DLL1 Assay
Product Specific Notices for Simple Plex Discovery Human DLL1 Assay
For research use only