Simple Plex Discovery Human Coagulation Factor III Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-012018
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human Coagulation Factor III Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 31 | 31 |
| Mean (pg/mL) | 31.4 | 1698 | 33.8 | 1625 |
| Standard Deviation | 3.97 | 68.3 | 3.62 | 132 |
| CV% | 7.2 | 4.0 | 10.7 | 8.1 |
Recovery for Simple Plex Discovery Human Coagulation Factor III Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 101 | 100-101 |
| EDTA Plasma (n=2) | 110 | 108-112 |
| Heparin Plasma (n=2) | 111 | 106-116 |
| Serum (n=2) | 96 | 93-99 |
Linearity
Samples containing and/or spiked with high concentrations of recombinant human Coagulation Factor III were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human Coagulation Factor III Assay
Simple Plex Discovery Human Coagulation Factor III/Tissue Factor Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: Coagulation Factor III/Tissue Factor
Coagulation Factor III, also known as Tissue Factor (TF), Thromboplastin, and CD142, is the primary initiator of the extrinsic coagulation pathway. It is a transmembrane protein that is consitutively expressed in subendothelial cells throughout the vasculature and is inducible on endothelial cells and monocytes. Damage to the vessel wall exposes Coagulation Factor III to circulating Factor VII, triggering the proteolytic cascade that results in fibrin clot formation. Coagulation Factor III plays an important role in thrombotic diseases, disseminated intravascular coagulation, and atherosclerosis. It also plays a role in non-hemostatic inflammation, angiogenesis, and tumor progression.
Alternate Names
Gene Symbol
Additional Coagulation Factor III/Tissue Factor Products
Product Documents for Simple Plex Discovery Human Coagulation Factor III Assay
Product Specific Notices for Simple Plex Discovery Human Coagulation Factor III Assay
For research use only