Simple Plex Discovery Human TSLP Assay
ProteinSimple, part of Bio-Techne | Catalog # SPCKB-PS-011826
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Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Discovery Human TSLP Assay Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Multiple replicates of each control were tested in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 25 | 27 |
| Mean (pg/mL) | 11.6 | 546 | 12.2 | 583 |
| Standard Deviation | 0.367 | 17.9 | 0.885 | 30.3 |
| CV% | 3.2 | 3.3 | 7.3 | 5.2 |
Recovery for Simple Plex Discovery Human TSLP Assay
Recovery at three different spiked concentrations within the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 110 | 107-113 |
| EDTA Plasma (n=2) | 86 | 84-87 |
| Heparin Plasma (n=2) | 99 | 96-102 |
| Serum (n=2) | 92 | 92-92 |
Linearity
Samples containing and/or spiked with high concentrations of recombinant human TSLP were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Scientific Data Images for Simple Plex Discovery Human TSLP Assay
Simple Plex Discovery TSLP Ella Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: TSLP
TSLP (Thymic Stromal Lymphopoietin) is a secreted cytokine that signals through a complex of TSLP R and IL-7 R alpha/CD127. It acts on thymic dendritic cells which convert potentially autoreactive T cells into regulatory T cells. TSLP promotes fetal liver pro-B cell proliferation as well as large pre-B cell differentiation and B cell lymphopoiesis in adult bone marrow. It is produced by allergen challenged keratinocytes and mucosal epithelial cells. It enhances Th2 biased allergic inflammation by limiting Th1/Th17 inflammation, promoting Th2 differentiation, and enhancing the function of basophils, eosinophils, and mast cells.
Long Name
Alternate Names
Gene Symbol
Additional TSLP Products
Product Documents for Simple Plex Discovery Human TSLP Assay
Product Specific Notices for Simple Plex Discovery Human TSLP Assay
For research use only